>
Fa   |   Ar   |   En
   In Vitro Transcription of Hiv-1 Rna For Standard Rna  
   
نویسنده Yasmon Andi ,Bela Budiman ,Ibrahim Fera ,Syahruddin Elisna
منبع Medical Journal Of Indonesia - 2011 - دوره : 20 - شماره : 3 - صفحه:185 -189
چکیده    Background: the quantitative assays are important tests in the management of patients with hiv-1/aids. the important step in developing the assay is the availability of the standard hiv-1 rna. for this purpose, we optimized in vitro hiv-1 rna transcription to produce the standard hiv-1 rna. methods: the hiv-1 dna was amplified from pnl43 by pcr using a primer pair that was specific for conserved region of hiv-1 gag gene. the pcr product was further cloned into pbluescript ii ks . the recombinant plasmid was restricted with ecori enzyme. then, the linearized plasmid was used as template for rna transcription. rt-pcr and pcr were performed simultaneously for confirmation of synthesized rna fragment. results: a 115 bp dna of hiv-1 gag gene has been cloned into pbluescript ii sk with the exact true orientation. the reaction of the rna transcription was also successfully performed. the rna transcripts have been confirmed and showed the accuracy of the transcripts. conclusion: we successfuly constructed the recombinant plasmid containing a conserved region of hiv-1 gag gene, and the hiv-1 rna has been transcribed in vitro as well. (med j indones. 2011; 20:185-9)
کلیدواژه Hiv-1/Aids ,Quantitative Assay ,Rna Transcription
آدرس University Of Indonesia, Faculty Of Medicine, Department Of Microbiology, Indonesia, University Of Indonesia, Faculty Of Medicine, Department Of Microbiology, Indonesia, University Of Indonesia, Faculty Of Medicine, Department Of Microbiology, Indonesia, University Of Indonesia, Faculty Of Medicine, Departement Of Pulmonology And Respiration Medicine, Indonesia
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved