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   Plasma Lipid Pattern and Red Cell Membrane Structure in Β-Thalassemia Patients in Jakarta  
   
نویسنده Freisleben Seruni K. U. ,Hidayat Jenny ,Freisleben Hans-Joachim ,Poertadji Soehardjo ,Kurniawan Budi ,Bo Na Peng. ,Handayani Sri ,Wahidiyat Pustika A. ,Soegianto Rondang R.
منبع Medical Journal Of Indonesia - 2011 - دوره : 20 - شماره : 3 - صفحه:178 -184
چکیده    Background: over the last 10 years, we have investigated thalassemia patients in jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage. methods: in blood samples from 15 transfusion-dependent patients (group t), 5 non-transfused patients (group n) and 10 controls (group c), plasma lipids and lipoproteins, lipid-soluble vitamin e, malondialdehyde (mda) and thiol status were measured. isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (epr) spectroscopy using doxyl-stearic acid and maleimido-proxyl spin lables. data were analyzed statistically with anova. results: plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. vitamin e, group c: 21.8 vs t: 6.2 μmol/l) and reactive thiols (c: 144 vs. t: 61 μmol/l) were considerably lower in transfused patients, who exert clear signs of oxidative stress (mda, c: 1.96 vs t: 9.2 μmol/l) and of tissue cell damage, i.e., high transaminases plasma levels. non-transfused thalassemia patients have slight signs of oxidative stress, but no signifi cant indication of cell damage. erythrocyte membrane parameters from epr spectroscopy differ considerably between all groups. in transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized. conclusion: in tranfusion-dependent thalassemic patients, plasma lipid pattern and oxidative stress are associated with structural damage of isolated erythrocyte membranes as measured by epr spectroscopy with lipid and proteinthiol spin labels. (med j indones 2011; 20:178-84)
کلیدواژه Electron Paramagnetic Resonance Spectroscopy ,Erythrocyte Membrane ,Lipoproteins ,Oxidative Stress ,Thalassemia ,Plasma Lipids.
آدرس University Of Indonesia, Faculty Of Medicine, Faculty Of Mathematics And Natural Sciences, Postgraduate Study Program Biomedical Sciences, Department Of Physics, Indonesia, University Of Indonesia, Faculty Of Medicine, Postgraduate Study Program Biomedical Sciences, Indonesia, University Of Indonesia, Faculty Of Medicine, Postgraduate Study Program Biomedical Sciences, Indonesia, University Of Indonesia, Faculty Of Mathematics And Natural Sciences, Department Of Physics, Indonesia, University Of Indonesia, Faculty Of Mathematics And Natural Sciences, Department Of Physics, Indonesia, University Of Indonesia, Faculty Of Mathematics And Natural Sciences, Department Of Physics, Indonesia, University Of Indonesia, Faculty Of Mathematics And Natural Sciences, Faculty Of Medicine, Department Of Chemistry, Postgraduate Study Program Biomedical Sciences, Indonesia, Dr. Cipto Mangunkusumo Hospital, Thalassemia Center, Department Of Child Health, Indonesia, University Of Indonesia, Faculty Of Medicine, Department Of Biochemistry And Molecular Biology, Indonesia
 
 

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