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   Development of Multiplex-Pcr Assay For Rapid Detection of Candida Spp.  
   
نویسنده Tarini Ni Made A. ,Wahid Mardiastuti H. ,Ibrahim Fera ,Yasmon Andi ,Djauzi Samsuridjal
منبع Medical Journal Of Indonesia - 2010 - دوره : 19 - شماره : 2 - صفحه:83 -87
چکیده    Aim candida spp. infection commonly occur in immunocompromised patients. biochemical assay for identification of candida spp. is time-consuming and shows many undetermined results. specific detection for antibody, antigen and metabolites of candida spp. had low sensitivity and specificity. in this study, we developed a rapid diagnostic method, multiplex-pcr, to identify candida spp. methods five candida spp. isolates were cultured, identified with germ tube and api® 20 c aux (biomerieux® sa) kit. furthermore, dna was purified by qiaamp dna mini (qiagen®) kit for multiplex-pcr assay. results dna detection limit by multiplex-pcr assays for c. albicans, c. tropicalis, c. parapsilosis, c. krusei and c. glabrata were 4 pg, 0,98 pg, 0,98 pg, 0,5 pg and 16 pg respectively. this assay was also more sensitive than culture in that multiplex-pcr could detect 2.6-2.9 x 100 cfu/ml, whereas culture 2.6-2.9 x 102 cfu/ml. conclusion multiplex-pcr is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of candida spp. (med j indones 2010; 19:83-7)
کلیدواژه Candida Spp. ,Multiplex-Pcr
آدرس University Of Udayana, Medical Faculty, Department Of Clinical Microbiology, Indonesia, University Of Indonesia, Faculty Of Medicine, Department Of Microbiology, Indonesia, University Of Indonesia, Medicine Faculty, Microbiology Department, Indonesia, University Of Indonesia, Faculty Of Medicine, Department Of Microbiology, Indonesia, University Of Indonesia, Faculty Of Medicine, Allergic-Immunology Division, Department Of Internal Medicine, Indonesia
 
 

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