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In Vitro Anti-Rotaviral Activity of Achillea Kellalensis
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نویسنده
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Taherkhani Reza ,Farshadpour Fatemeh ,Makvandi Manoochehr
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2013 - دوره : 8 - شماره : 3 - صفحه:138 -143
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چکیده
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Background: achillea kellalensis,which is frequently used by chaharmahal va bakhtiarians residing in,southwest of iran,as a traditional herbal medicine for the treatment of acute diarrhea,has been selected to examine its antiviral activities against bovine rotavirus and cell toxicity activity in ma-104 cells. objectives: the aim of this study was to evaluate the in vitro cytotoxic and anti-rotavirus properties of crude extracts of a. kellalensis. materials and methods: the dried and powdered flowers of achillea kellalensis were extracted with hot water and ethanol 50% (v/v). the cell viability and toxicity of the extracts were evaluated on ma-104 cells using four methods; trypan blue dye,nr,crystal violet and mtt assay. the in vitro anti-rotavirus properties were determined via four different assays,in order to evaluate the direct inhibition and/or the inhibition of viral replication. results: cytotoxicity of two a. kellalensis extracts showed different concentrations. hydro-alcoholic extract had low cc50 at 600 μg/ml by the nr assay while the aqueous extract had high cc50 at 1000μg/ml by the crystal violet method. in the simultaneous treatment assay and post treatment assay,the extracts were able to prevent viral replication and inhibit the viral cpe on ma-104 cells at 10 tcid50,but the extracts did not exhibit direct antiviral activity on rotavirus adsorption. the effective concentration (ec50) of both extracts was observed to be 100 μg/ml. conclusions: these results indicate that a. kellalensis extracts exert potent anti-rotaviral activity only after viral adsorption. the two extracts from a. kellalensis showed a good selectivity index. also these results suggest that extracts prepared from the flowers of a. kellalensis may be potential anti-rotaviral agents in vivo and be useful in veterinary medicine. © 2013,school of pharmacy,ahvaz jundishapur university of medical sciences; licensee docs ltd.
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کلیدواژه
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Achillea; Cell Survival; In Vitro
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آدرس
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Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران
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Authors
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