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Relationship Between Cell Compatibility and Elastic Modulus of Siliconerubber/Organoclay Nanobiocomposites
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نویسنده
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Sadat Hosseini Motahare ,Tazzoli-Shadpour Mohammad ,Amjadi Issa ,Haghighipour Nooshin ,Shokrgozar Mohammad Ali ,Ghafourian Boroujerdnia Mehri
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2012 - دوره : 7 - شماره : 2 - صفحه:65 -70
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چکیده
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Substrates in medical science are hydrophilic polymers undergoing volumeexpansion when exposed to culture medium that influenced on cell attachment.although crosslinking by chemical agents could reduce water uptake and promote mechanicalproperties, these networks would release crosslinking agents. in order to overcomethis weakness, silicone rubber is used and reinforced by nanoclay.objectives: attempts have been made to prepare nanocomposites based on medicalgrade htv silicone rubber (sr) and organo-modified montmorillonite (ommt) nanoclaywith varying amounts of clay compositions.materials and methods: incorporation of nanocilica platelets into sr matrix was carriedout via melt mixing process taking advantage of a brabender internal mixer. thetensile elastic modulus of nanocomposites was measured by performing tensile tests onthe samples. produced polydimetylsiloxane (pdms) composites with different flexibilitiesand crosslink densities were employed as substrates to investigate biocompatibility,cell compaction, and differential behaviors.results: the results presented here revealed successful nanocomposite formation withsr and ommt, resulting in strong pdms-based materials. the results showed that viability,proliferation, and spreading of cells are governed by elastic modulus and stiffnessof samples. furthermore, adipose derived stem cells (adscs) cultured on pdms andcorresponding nanocomposites could retain differentiation potential of osteocytes inresponse to soluble factors, indicating that inclusion of ommt would not prevent osteogenicdifferentiation. moreover, better spread out and proliferation of cells was observedin nanocomposite samples.conclusions: considering cell behavior and mechanical properties of nanobiocompositesit could be concluded that silicone rubber substrate filled by nanoclay are a goodchoice for further experiments in tissue engineering and medical regeneration due toits cell compatibility and differentiation capacityimplication for health policy/practice/research/medical education:improved mechanical properties of nanobiocomposites result in proper cell response through adjustment and arrangement ofcytoskeletal fibers. results can be applied in tissue engineering when enhanced substrates are required for in vivo improvementof cell behavior.
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کلیدواژه
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Nanocomposite ,Elastic Modulus ,Cytotoxicity Tests
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آدرس
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Amirkabir University Of Technology, Biomedical Group, Faculty Of Biomedical Engineering (Center Of Excellence), Amirkabir University Of Technology, Tehran, Ir Iran, ایران, Amirkabir University Of Technology, Biomedical Group, Faculty Of Biomedical Engineering (Center Of Excellence), Amirkabir University Of Technology, Tehran, Ir Iran, ایران, Amirkabir University Of Technology, Biomedical Group, Faculty Of Biomedical Engineering (Center Of Excellence), Amirkabir University Of Technology, Tehran, Ir Iran, ایران, Pasteur Institute Of Iran, Tehran, Ir Iran, National Cell Bank Of Iran, Pasteur Institute Of Iran, Tehran, Ir Iran, ایران, Pasteur Institute Of Iran, Tehran, Ir Iran, National Cell Bank Of Iran, Pasteur Institute Of Iran, Tehran, Ir Iran, ایران, Ahvaz Jundishapur University Of Medical Sciences, Immunology Department, Medical College, Ahvaz Jundishapur University Of Medical Science, Ahvaz, Ir Iran, ایران
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Authors
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