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Nanosilver Particle Production Using Juglans Regia L.(Walnut) Leaf Extract
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نویسنده
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Sadat Jalayer Susan ,Korbekandi Hassan ,Asghari Gholamreza ,Jalayer Maryam Sadat ,Bandegani Maedeh
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2013 - دوره : 8 - شماره : 1 - صفحه:20 -26
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چکیده
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Background: the production of nanoparticles using a biosystem is considered green chemistry. application of plant extracts as a biologicalprocess has been proven to be suitable for synthesis of nanoparticles. objectives: this study designed in order to evaluate the production of silver nanoparticles using juglans regia leaf extract and to compare theoutcome of different preparation methods of plant extracts (ethanolic extract, boiling water extract and plant powder) for the generation ofnanoparticles. materials and methods: the reaction mixture contained the following ingredients: agno3 (10 mm) as the biotransformation substrate, plantextract or powder as the biocatalyst, glucose (560 mm) as the electron donor, phosphate buffer (ph = 7, 100 mm) and ethanol 70% as the solventin the reaction mixture. the samples were taken from the reaction mixtures at different times, and the absorbance (450 nm) of the colloidalsuspensions of silver nanoparticle hydrosols was recorded immediately following dilution (1:80) so as to preserve its freshness. results: uv-visible spectrophotometer analysis revealed that the direct application of powder of the walnut leaf was the most efficienttechnique. tem (transmission electron microscopy) micrograph obtained by using this method revealed the generation of aggregatedpolydisperse, quasi-spherical nanoparticles in sizes of 10-50 nm. ethonolic extract resulted in single silver nanoparticles which were nearlymonodisperse, spherical, and individual nanoparticles ranged in size from 1-5 nm. therefore, using direct powder of walnut created moreparticles but applying ethanolic extract synthesized particles with smaller dimensions and no aggregation. conclusions: different preparation methods of juglans regia influence silver nanoparticles formation.
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کلیدواژه
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Silver ,Nanoparticles ,Biosynthesis ,Plant Extract
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آدرس
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Payame Noor University, Department Of Biotechnology, Karaj Payam Noor University, Karaj, Ir Iran, ایران, Isfahan University Of Medical Sciences, Department Of Genetics & Molecular Biology, School Of Medicine, Isfahan University Of Medical Sciences, Isfahan, Ir Iran, ایران, Isfahan University Of Medical Sciences, Isfahan Pharmaceutical Sciences Research Center, School Of Pharmacy, Isfahan University Of Medical Sciences, Isfahan, Ir Iran, ایران, Tarbiat Modares University, Department Of Science, Tarbiat Modares University, Tehran, Ir Iran, ایران, Payame Noor University, Department Of Biotechnology, Karaj Payam Noor University, Karaj, Ir Iran, ایران
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Authors
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