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The Effects of Quercetin on the Gene Expression of Arginine Metabolism Key Enzymes in Human Embryonic Kidney 293 Cells
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نویسنده
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Jamehdor Saleh ,Farivar Shirin ,Sangtarash Mohammad Hossein ,Amini Razieh ,Pajouhanfar Sara ,Teimoori Ali
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2021 - دوره : 16 - شماره : 2 - صفحه:1 -7
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چکیده
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Background: arginine metabolism is an important factor involved in tumorigenesis, progression, and survival of tumor cells. besides, other metabolites produced in the arginine metabolism process, such as polyamines, nitric oxide, argininosuccinate, and agmatine, play key roles in different stages of tumor development. on the other hand, herbal metabolites are widely used to treat cancer. one of these herbal flavonoids is quercetin. methods: in this study, according to mtt assay data, two concentrations of quercetin flavonoid were selected (57.5 and 115 µm) to treat human embryonic kidney 293 (hek293) cells. then rna was extracted from the cells and used as a template for cdna synthesis. using real-time pcr, the expression of key enzymes involved in arginine metabolism was evaluated, including arginase 2 (arg2), ornithine carbamoyl transferase (otc), agmatinase (agmat), arginase 1 (arg1), nitric oxide synthase 1 (nnos), arginine decarboxylase (adc), ornithine decarboxylase 1 (odc), ornithine carbamoyl transferase (oct), spermidine synthase (srm), spermine synthase (sms), argininosuccinate synthase 1 (ass1), and argininosuccinate lyase (asl). the student t-test was used to analyze the data considering a p value of < 0.05 as the significance level. results: our results indicated significant changes in the expression of arginine metabolism enzymes after quercetin exposure, confirming a role for quercetin plant flavonoid in regulating arginine metabolism in hek293 cells. conclusions: quercetin could alter the gene expression of the key enzymes involved in arginine metabolism. this was the first study investigating the effects of quercetin on arginine metabolism in hek293 cells.
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کلیدواژه
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Arginine ,Cancer ,Hek293 Cell ,Quercetin ,Polyamine
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آدرس
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University Of Sistan And Baluchestan, Department Of Biology, Iran, Shahid Beheshti University, Faculty Of Life Sciences And Biotechnology, Department Of Cell And Molecular Biology, Iran, University Of Sistan And Baluchestan, Department Of Biology, Iran, Hamadan University Of Medical Sciences, School Of Medicine, Department Of Molecular Medicine, Iran, Iran University Of Medical Sciences, Department Of Medical, Iran, Hamadan University Of Medical Sciences, Faculty Of Medicine, Department Of Virology, Iran
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پست الکترونیکی
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teimooriali1982@gmail.com
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Authors
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