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Anti-Mutagenicity and Apoptotic Effects of Teucrium Polium L. Essential Oil in Ht29 Cell Line
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نویسنده
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Hashem-Dabaghian Fataneh ,Shojaii Asie ,Asgarpanah Jinous ,Entezari Maliheh
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2020 - دوره : 15 - شماره : 3 - صفحه:1 -9
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چکیده
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Objectives: regarding the high prevalence of cancer in iran and the cytotoxic properties of t. polium, the current study aimed to investigate the cytotoxic and antimutagenicity effect of t. polium essential oil (tpeo) on human colon adenocarcinoma cell line (ht29). methods: ht29 cells were cultured in lglutamine, rpmi sigma (1640), with 10% of fbs (fetal bovine serum). then, the cultures were incubated with 5% co2 at 37°c, and mtt assay was used to recognize cell proliferation under the inhibitory influence of tpeo. the cell cycle progression was monitored by subg1 apoptosis assay using flow cytometry. eventually, the antimutagenicity property was evaluated using the ames test employing ta100 and exposure to sodium azide as the carcinogenic substance. results: the cytotoxic effect of tpeo on ht29 cells was 66.867 ± 1.37 µg/ml. cultured ht29 cells treated with tpeo exhibited morphological features of apoptosis. tpeo preventive effect was about 78.18%. conclusions: this study showed that tpeo may be useful for treating colon cancer.
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کلیدواژه
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Teucrium Polium ,Persian Medicine ,Herbal Medicine ,Ht29 Cell Line ,Mutagenicity ,Anti-Neoplastic Agents
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آدرس
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Iran University Of Medical Sciences, Research Institute For Islamic And Complementary Medicine, School Of Persian Medicine, Iran, Iran University Of Medical Sciences, Research Institute For Islamic And Complementary Medicine, School Of Persian Medicine, Iran, Islamic Azad University, Tehran Medical Sciences Branch, Herbal Pharmacology Research Center, Iran, Islamic Azad University, Tehran Medical Sciences Branch, Department Of Medical Biotechnology, Iran
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پست الکترونیکی
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mentezari@iautmu.ac.ir
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Authors
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