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The Effects of Doxorubicin, Ethanol Extract and Flavonoid-Rich Fraction of Euphorbia Splendida Mobayen on the Parp Level, and Apc Gene Expression in Ht-29 Human Colon Cancer Cell Line
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نویسنده
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Aein Mojdeh ,Fallah Soudabeh ,Ahmadpour Fatemeh ,Dabili Sheyda ,Shojaii Asie ,Fadaei Reza
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2020 - دوره : 15 - شماره : 3 - صفحه:1 -8
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چکیده
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Background: natural products derived from various sources are being used to develop chemotherapeutic drugs. euphorbiaceae is widely used to treat different types of cancers. colorectal cancer (crc) is the second and third cause of cancer in women and men, respectively. crc is strongly associated with the deregulation of the adenomatous polyposis coli (apc) gene and poly [adpribose] polymerase (ppar) protein. objectives: the current study aimed to examine the effect of doxorubicin, ethanol extract, and the flavonoidrich fraction of euphorbia splendida mobayen on colon cancer ht29 cell line death, apc gene expression, and ppar concentration. methods: following treatment of cells by euphorbia ethanol extract, euphorbia flavonoidrich fraction, and doxorubicin, cell viability assay was used to investigate the viability status of the ht29 cell line. total rna was isolated from the cell line and converted into cdna. the expression level of the apc gene was determined by quantitative realtime pcr. poly (adpribose) polymerase (ppar) protein was detected by the eliza method. results: we found that euphorbia ethanol extract, euphorbia flavonoidrich fraction, and doxorubicin can stimulate dosedependent cell death in the ht29 cell line, increase acp gene expression (p = 0.001, p = 0.041, p = 0.019), and decrease parp level (p = 0.001, p = 0.001, p = 0.001, respectively). conclusions: the findings indicated that doxorubicin, ethanol extract, and the flavonoidrich fraction of euphorbia splendida mobayen had cytotoxic effects on human colon cancer ht29 cell line by possibly stimulating apoptosis.
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کلیدواژه
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Cell Death ,Doxorubicin ,Flavonoid ,Ht-29 Cells ,Colon Cancer
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آدرس
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Iran University Of Medical Sciences, Faculty Of Medicine, Department Of Biochemistry, Iran, Iran University Of Medical Sciences, Faculty Of Medicine, Department Of Biochemistry, Iran, Iran University Of Medical Sciences, Faculty Of Medicine, Department Of Biochemistry, Iran, Iran University Of Medical Sciences, Faculty Of Medicine, Department Of Biochemistry, Iran, Iran University Of Medical Sciences, Research Institute For Islamic And Complementary Medicine, School Of Traditional Medicine, Iran, Tehran University Of Medical Sciences, Faculty Of Medicine, Department Of Biochemistry, Iran
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پست الکترونیکی
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reza.fadaei19@gmail.com
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Authors
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