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Effect of Epigenetic Drug Candidate Olsalazine on the Expression of Cdh1 and Upa Genes in Mcf-7 Breast Cancer Cell Line
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نویسنده
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Naghitorabi Mojgan ,Saki Ghasem ,Gharishvandi Sedighe
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منبع
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Jundishapur Journal Of Natural Pharmaceutical Products - 2020 - دوره : 15 - شماره : 4 - صفحه:1 -6
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چکیده
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Background: a main epigenetic change in cancer is dna methylation, which leads to the inactivation of tumor suppressor genes. due to its reversible nature, many studies have focused on how to correct epigenetic imbalances via inhibiting dna methyltransferases (dnmts). recent studies have shown that olsalazine can be a potent candidate for dnmt inhibition. objectives: the current study aimed to assess the cytotoxic effect of olsalazine on mcf7 cells and the expression of cdh1 and upa, as cancerrelated genes, compared to decitabine. methods: the cytotoxicity of olsalazine and decitabine on mcf7 cells was assessed by mtt assay. to evaluate the effect of drugs on the expression of cdh1 and upa genes, mcf7 cells were treated with olsalazine and decitabine in concentrations below their ic50 values. after 24 h, rna of treated cells was extracted and then subjected to a quantitative reverse transcriptionpolymerase chain reaction (qrtpcr).results: the mtt assay showed that olsalazine was more toxic (ic50 = 1.75 mm) in mcf7 cells than decitabine (ic50 = 3mm). qrtpcr analysis showed that olsalazine can significantly increase upa expression along with a nonsignificant increase in cdh1 expression. meanwhile, no significant change was found in gene expression after treatment with decitabine. conclusions: this study demonstrated that olsalazine was more cytotoxic than decitabine on mcf7 cells. also, compared to decitabine, olsalazine could increase the expression of cdh1 and upa genes. it suggests that olsalazine might be more potent than decitabine in inhibiting dnmts, although further studies are needed.
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کلیدواژه
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Olsalazine ,Epigenetic ,Dna Methyltransferase ,Cdh1 ,Upa
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آدرس
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Ahvaz Jundishapur University Of Medical Sciences, Cellular And Molecular Research Center, School Of Pharmacy, Department Of Pharmacognosy, Iran, Ahvaz Jundishapur University Of Medical Sciences, Cellular And Molecular Research Center, Iran, Ahvaz Jundishapur University Of Medical Sciences, School Of Pharmacy, Department Of Pharmacognosy, Iran
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پست الکترونیکی
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s.ghoreishvandi1999@gmail.com
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Authors
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