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Low Frequency of C-Mpl Gene Mutations in Iranian Patients With Philadelphia-Negative Myeloproliferative Disorders
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نویسنده
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Ghotaslou A. ,Nadali F. ,Chahardouli B. ,Alizad Ghandforosh N. ,Rostami Sh. ,Alimoghaddam K. ,Ghavamzadeh A.
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منبع
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Iranian Journal Of Pediatric Hematology And Oncology - 2015 - دوره : 5 - شماره : 1 - صفحه:43 -49
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چکیده
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Background: myeloproliferative disorders are a group of diseases characterized by increased proliferation of myeloid lineage. in addition to jak2v617f mutation, several mutations in the c-mpl gene were described in patients with philadelphia-negative chronic myeloproliferative disorders that could be important in the pathogenesis of diseases. the aim of present study was to investigate the frequency of c-mpl and jak2v617f mutations in iranian patients with philadelphia-negative myeloproliferative disorders. material and methods: peripheral blood samples from 60 patients with philadelphia-negative mpd) subgroups et and pmf) and 25 healthy subjects as control were collected in order to investigate the mutation status of c-mpl and jak2v617f by using amplification-refractory mutation system (arms) and allele-specific pcr (as-pcr), respectively and results were confirmed by sequencing. results: among the total 60 patients studied, 34 (56.6%) and 1(1.7%) had jak2v617f and c-mpl mutation, respectively. patients with jak2v617f mutation had higher wbc counts and hemoglobin concentration than those without the mutation (p= 0.005, p=0.003). in addition for all healthy subjects in control group, mutation was negative. conclusions: the present study revealed that the c-mpl mutations unlike the jak2v617f mutations were rare in iranian patients with ph-negative mpns and the low mutation rate should be considered in the design of screening strategies of mpd patients.
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کلیدواژه
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Arms –Pcr ,C- Mpl Mutation ,Jak2v617f ,Myeloproliferative Disorders
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آدرس
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Tehran University Of Medical Sciences Tums, School Of Allied Medical Sciences, Department Of Hematology, ایران, Tehran University Of Medical Sciences Tums, School Of Allied Medical Sciences, Department Of Hematology, ایران, Tehran University Of Medical Sciences Tums, Hematology-Oncology And Stem Cell Transplantation Research Center, ایران, Tehran University Of Medical Sciences Tums, Hematology-Oncology And Stem Cell Transplantation Research Center, ایران, Tehran University Of Medical Sciences Tums, Hematology-Oncology And Stem Cell Transplantation Research Center, ایران, Tehran University Of Medical Sciences Tums, Hematology-Oncology And Stem Cell Transplantation Research Center, ایران, Tehran University Of Medical Sciences Tums, Hematology-Oncology And Stem Cell Transplantation Research Center, ایران
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Authors
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